Structures & laboratories

PEPTLAB@UNIFI

The Research Units PeptLab@Unifi is located in the Department of Chemistry "Ugo Schiff" and in the Department NEUROFARBA (Department of Neurosciences, Psychology, Drug Research and Child Health) of the University of Florence in Sesto Fiorentino.

PeptLab@Unifi is a multidisciplinary Research Units with a strong know-how in the field of peptide and protein, particularly in their synthesis and molecular characterization, both at the biological and structural level. In fact this Research Units integrates competences and accessible facilities with hightech instrumentation dedicated to synthetic and analytical chemistry, immunology/biology and clinics aimed to Research & Development of innovative diagnostics of "difficult diseases".

With the term "difficult diseases" we refer to those diseases (rare but not only) for which up to now clinicians have no or few tools to characterize them.

PeptLab@Unifi: Polo Scientifico via della Lastruccia, 13 and Via U. Schiff, 6
50019, Sesto Fiorentino, Firenze (Italy)

PeptLab@Unifi Platform’s equipments

Peptide Synthesis

Manual parallel peptide synthesizer and Semi Automatic peptide synthesizer

Semi Automatic Synthesizer SAS (MultiSyntech)
Automatic Peptide Synthesizer PS-3 (Protein Technology)
Multiple Solid Phase Organic Synthesizer with temperature control PLS 4x4 (Advanced ChemTech)

 

Automatic peptide synthesizer

Multiple-Peptide Automatic Synthesizer APEX 396 (Advanced ChemTech)
Automated microwave peptide synthesizer Liberty (CEM)  
Microwave Synthesizer Explorer S Class (CEM)  

 

Purification and characterization

Flash Chromatography Isolera One (Biotage)  
SPE Solid Phase Extraction: Flash Chromatography Spot (Armen Instruments)  
HPLC system coupled with a mass detector for peptide characterization: Mass Spectrometer LC-ESI et APCI, LCQ Advantage (Thermo Finnigan)  
Analytical Alliance HPLC System with auto-sampler coupled with a mass detector for peptide characterization: MS spectrometer Micromass ZQ (Waters)  
ACQUITY™ UPLC system (Waters) coupled with a Micromass® Q-Tof MICROTM mass spectrometer (Waters) equipped with an ESI source  
HPLC system: RP-HPLC Analytical and semi preparative Alliance HPLC System with auto-sampler (Waters)

Recombinant Proteins Production by Molecular Biology

Laminar flow hood (Heraeus) and Laminar flow hood mod “ratio” (PBI)
AktaBasic Liquid Chromatography System (GE Healthcare)  
Allegra 64R Centrifuge (Beckman Coulter)  

Biomarkers Immunochemical characterization

Multi-wells ELISA washing machine, Columbus (Tecan)
Multi-wells ELISA UV-Spectrophotometer, SUNRISE (Tecan)
Biacore T100 (GE Healthcare)

Getting to PeptLab@Unifi

Arriving by car

Going in the direction of the Highway A11 (Firenze/Mare) and the Airport of Florence, follow the pointers to "Polo Scientifico e Tecnologico", go on through via dell' Osmannoro and turn on the right at the Pointer "Polo Scientifico e Tecnologico", access from Viale delle Idee.

Arriving by train

Florence Rifredi train station ATAF Bus no. 59 (each 20 minutes).

Arriving by plane

The airport Amerigo Vespucci is located 5 minuts by car from the Scientific Pole.