Projects / Diagnostics to detect antibodies in patient sera / Peptide based diagnostics
Reliable diagnostic devices based on synthetic peptides
- Anna Maria PAPINI
- Paolo ROVERO
- Maud LARREGOLA
- Elisa PERONI
In collaboration with
Ecole Normale Supérieure, Département de Chimie,UMR CNRS-ENS-UPMC 8640 PASTEUR, Paris France.
Catherine Sella and Laurent Thouin
Maria Claudia Alcaro and Ilaria Paolini
Recognition of specific antibodies present in sera of patients affected by diseases involving an immune response, e.g. autoimmune disorders and infections, is a relevant goal because these antibodies may have a diagnostic or prognostic value. Indeed, diagnostics for immune-related diseases still represent an unmet medical need. To this aim optimized peptide structures (Synthetic Antigenic Probes) have been selected starting from the idea that antibodies recognise an epitope (both linear and/or conformational) including no more than 8-10 amino acids and most importantly the post-translational modification(s) possibly reminiscent of a bacterial/viral infection. Therefore these synthetic antigenic probes are combined to newly developed label-free diagnostic devices.
These innovative devices are based on Surface Acoustic Wave technique, or on amperometric techniques performed in microfluidic channels.
Preliminary tests  with the electrochemical detection using human sera demonstrated the feasibility of this approach with fast and semi-quantitative evaluations. These data can demonstrate the ability to selectively detect (auto)antibodies. In this context, an oxidation mechanism of the SAP in the presence of (auto)antibodies was proposed to report peptide-antibody interactions and explain the electrochemical detection performances achieved.
On the other hand Surface Acoustic Wave technology is based on biosensors chip for real-time measurements of biomolecular interactions, especially for peptide-antibody interactions kinetics. As the signal inherently shows little influence of bulk effects, making this technology ideal to detect specific antibodies in impure samples. Additionally, it can give structural information thereby allowing the differentiation between IgM and IgG antibodies as unpublished measurements suggested Moreover in previous setups it has been shown that the SAW technology is faster than ELISA
Thèse de Doctorat, UNIVERSITÉ PIERRE ET MARIE CURIE « Détection d’auto-anticorps biomarqueurs de la sclérose en plaques : Etude et caractérisations électrochimiques d’interactions peptides-anticorps » :Wided BELLAGHA-CHENCHAH, 14-03-2013.